Pierce ECL Plus Western Blotting Substrate characteristics include: Sensitivity: low. Part of The Investigator's Annexe program. Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. The superoxides then convert the luminol to aminothalate ions, which gives rise to the chemiluminescence light at 430nm for visual detection of your protein.įrom the laboratory of Michael A. PI80196X3) through Fisher Scientific and other channels. This product is sold as Pierce ECL 2 Substrate (Cat. The chemiluminescent (CL) reaction of luminol (the active ingredient in the buffer) is catalyzed by superoxide anions which are produced emzymatically from H 20 2. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. The most popular method used to visualize a protein at the nanogram level involves enhanced chemiluminescence or ECL. ECL(TM) Western Blotting Detection Reagents in combination with Hybond ECL(TM) membranes form a complete solution for Western blot applications.Emission. The technique uses three elements: (1) separation by size, (2) transfer to a solid support, and (3) marking the target protein with specific primary and secondary antibody to visualize. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence. Pierce ECL Western Blotting Substrate (when used horseradish. The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The high signal intensity can fully utilize the. Western Blot Analysis The study of the expression of the main proteins involved in the. Western blot is a widely used analytical technique to detect specific proteins from a complex mixture of proteins extracted from cells or tissue. This makes Amersham ECL Select suitable for the most demanding Western blots regarding sensitivity requirements. Increased signal stability over time (~3hrs).Faster exposure times (10-20 sec, compared to 2+ min) Bosters ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase. ![]() Increased sensitivity and signal, compared to other commercial ECL reagents.Buffered ECL reagent with optimized pH, provides a stronger and more stable chemiluminescent signal for western blots, compared to other commercial ECL reagents. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background We compared several alternative ECL solutions for Western blot detection of endogenous proteins in whole cell lysates using inexpensive, commercially available reagents.
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